28(1)143-146 中国生物防治学报 Chinese Journal of Biological Control 2012 年2月收稿日期:2011-04-13
基金项目:广东省科技成果转化启动项目(2010B060600008);广州市科技计划项目(2006Z3-E0711)
作者简介:刘桂清(1981),女,硕士,助理研究员,E-mail:pepsiliu81@163.com;*通讯作者,硕士,高级工程师,E-mail:gdipml@gdei.gd.cn.
利用昆虫病原线虫防治草菇双额岩小粪蝇
刘桂清,徐洁莲,欧剑峰,吴华,黄鸿*
(广东省昆虫研究所,广州 510260)
摘要:草菇双额岩小粪蝇 Bifronsina bifrons 是当前草菇生产中的重要害虫.本文测定了 5 个线虫品系在(30±0.5)
℃下对草菇双额岩小粪蝇的致死率和 LT50.结果表明,各线虫品系在浓度 4000 IJsmL1
下的 LT50 范围为 2.13~
4.71 d,其中夜蛾斯氏线虫 Steinernema feltiae SN 品系对双额岩小粪蝇的毒力最大,LT50 为2.13 d,侵染 5 d 后
的致死率达 86.67%.菇房施用 4*106
IJs.m2
S. feltiae SN 品系和嗜菌异小杆线虫 Heterorhabditis bacteriophora
H06 品系悬浮液于培养基表面 6 d 后,对双额岩小粪蝇的防治效果分别达到 70.37%和45.68%,SN 线虫对双
额岩小粪蝇的防效与阿维菌素相当.
关键词:昆虫病原线虫;双额岩小粪蝇;生物防治;草菇
中图分类号:S436.462;S476.15 文献标识码:A 文章编号:1005-9261(2012)01-0143-04
Using Entomopathogenic Nematodes for Control of Mushroom Fly, Bifronsina bifrons
(Stenhammar) (Diptera: Sphaeroceridae)
LIU Guiqing, XU Jielian, OU Jianfeng, WU Hua, HUANG Hong*
(Guangdong Entomological Institute, Guangzhou 510260, China)
Abstract: Mushroom fly, Bifronsina bifrons, is an important insect pest in straw mushroom production in South
China. Virulence of five strains of Steinernema and Heterorhabditis to the fly was bioassayed at (30±0.5) ℃ in
laboratory. Results showed that LT50 values of the five strains to the mushroom fly ranged from 2.13 d to 4.71 d. S.
feltiae SN strain was a potential biocontrol agent against the mushroom fly with LT50 value of 2.13 d and 86.67% of
lethality at the concentration of 4000 IJsmL1
at 5 d after treatment. Field trails demonstrated that when nematode
suspensions containing 4*106
IJsm2
were sprayed onto the mushroom beds the day before inoculation, control
efficacy of S. feltiae SN strain and H. bacteriophora H06 strain against the mushroom fly at 6 d after treatment was
70.37% and 45.68%, respectively, and the control efficacy of S. feltiae SN strain was almost the same as that of

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